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Visualizing virus replication in 3 dimensions

07.05.2009
Scientists at Heidelberg University Hospital present the first three-dimensional model of dengue virus replication / Article in Cell Host & Microbes

Dengue fever is the most common infectious disease transmitted by mosquitoes – some 100 million people around the world are infected. Researchers at the Hygiene Institute at Heidelberg University Hospital were the first to present a three-dimensional model of the location in the human cell where the virus is reproduced. Their research provides an insight into the exact process of viral replication and serves as a model for other viruses whose replication is still unclear, such as the hepatitis C virus. In addition, it offers new approaches for developing measures to prevent or treat dengue fever. Up to now, neither a vaccine nor a specific antiviral therapy exists. 

 

Professor Dr. Ralf Bartenschlager, director of the Department of Molecular Virology at the Heidelberg Hygiene Institute and his team, working in cooperation with colleagues from the European Molecular Biology Laboratory (EMBL) have published their study in the latest issue of the prestigious journal Cell Host & Microbes.

 

Viruses do not have a metabolism and cannot produce proteins from their genetic material (RNA or DNA) on their own. They can replicate only inside a host cell – but where and how exactly does this take place? The answer to this question is crucial for developing therapy.

 

Viruses transform human cell membranes for their purposes

 

Dengue viruses reproduce in what is known as the endoplasmic reticulum, a membrane network interconnected with the nuclear envelope; this is where proteins are synthesized. The dengue virus uses this membrane network and transforms it for its own use. 

 

"We now know that viral RNA is replicated in vesicles in the endoplasmic reticulum and is secreted through tiny pores. We were also able to show that replication of the virus genome and its encapsulation in new virus particles are directly linked,” said Professor Bartenschlager. The new virus genomes are secreted through pores into the intracellular space where they are incorporated into pre-stages of viruses and then penetrate the endoplasmic reticulum a second time. There they are enveloped in a membrane that disguises them for the cell so that they can be secreted like normal cellular material. The reproduction cycle can begin again.

 

 

References:

Sonja Welsch, Sven Miller, Ines Romero-Brey, Andreas Merz, Christopher

Bleck, Paul Walther, Stephen D. Fuller, Claude Antony, Jacomine Krijnse-Locker, Ralf

Bartenschlager, Composition and Three-Dimensional Architecture of the Dengue Virus Replication and Assembly Sites, Cell Host & Microbes 2009, 5, 4.

 

 

Internet:

www.klinikum.uni-heidelberg.de/DENGUE.104918.0.html

 

 

[show in higher resolution]

 

Caption: Cover illustration of the newest issue of Cell Host & Microbes. In the background in gray is a normal, two-dimensional image of the virus on an electron microscope. The 3D model is superimposed. The tubules of the endoplasmic reticulum and inside them the balloon-like vesicles where the dengue virus replicates its genome can be seen. Image source: Hygiene Institute at Heidelberg University Hospital.

 

Contact person:

Prof. Dr. Ralf Bartenschlager

Department of Molecular Virology

Heidelberg University School of Medicine

Im Neuenheimer Feld 345

69120 Heidelberg

Tel.: 06221 56-4569

E-mail: Ralf_Bartenschlager@med.uni-heidelberg.de

Internet: http://www.molecular-virology.uni-hd.de

 

 

 

Heidelberg University Hospital and Medical Faculty:

Internationally recognized patient care, research, and teaching 

Heidelberg University Hospital is one of the largest and most prestigious medical centers in Germany. The Medical Faculty of Heidelberg University belongs to the internationally most renowned biomedical research institutions in Europe. Both institutions have the common goal of developing new therapies and implementing them rapidly for patients. With about 7,000 employees, training and qualification is an important issue. Every year, around 500,000 patients are treated on an inpatient or outpatient basis in more than 40 clinics and departments with 1,600 beds. Currently, about 3,100 future physicians are studying in Heidelberg; the reform Heidelberg Curriculum Medicinale (HeiCuMed) is one of the top medical training programs in Germany.

 

Requests by journalists:

Dr. Annette Tuffs

Head of Public Relations and Press Department

University Hospital of Heidelberg and

Medical Faculty of Heidelberg

Im Neuenheimer Feld 672

D-69120 Heidelberg

Germany

phone: +49 6221 / 56 45 36

fax: +49 6221 / 56 45 44

e-mail: annette.tuffs(at)med.uni-heidelberg.de

 

 

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