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Molecular Medicine Partnership Unit

 

Project descriptions JCF call October 2018

Offered dissertation projects are displayed here.

 

Project in Müller-Tidow Group

Project leaderProf. Dr. med. Carsten Müller-Tidow
Project supervisor: Dr. med. Caroline Pabst
Affiliation:

Med. Klinik V, University Hospital Heidelberg

MMPU

Start of MD project: tbd


Project description: 
Title:

AML- Bone marrow niche-haematopoietic stem cell interactions
Summary:

Long-term survival rates in AML patients, especially in those of advanced age, are very low. Hence the need for new, effective, less toxic therapeutic options. The bone marrow niche (BM), specifically its interactions with AML blasts, has been identified as a major player in the development of chemoresistance and relapse. Thus, research into this area has great potential therapeutic value.

The goal of this dissertation is to contribute to the elucidation of BM-haematopoetic stem cell (HSC) interactions with respect to their relevance in leukaemia development, maintenance of leukaemia stem cells (LSCs), and chemoresistance. In particular, the aim is to investigate the role of specific candidate genes in BM-HSC/LSC interactions, and asses their theoretical potential as therapeutic targets.

Methods:

Ex vivo culture and manipulation (genetic engineering) of primary Human HSCs; from different sources including cord blood, mobilised peripheral blood, bone marrow, AML patient cells, model leukaemias, cell lines. Virus production, knock-down and overexpression of genes in these cells, CRSIPR/Cas9 technology, Flow cytometry and immunofluorescence, Xenotransplantation.

For more information please visit our websites:

MMPU Research Group Stem-Cell-Niche-Networks
AG Müller-Tidow
AG Pabst

Contact:

Caroline.Pabst@med.uni-heidelberg.de

 

 

 

Project in Kulozik Group

Project leaderProf. Dr. Andreas Kulozik
Project supervisor: Prof. Dr. Andreas Kulozik
Affiliation:

Zentrum für Kinder- und Jugendmedizin, Pädiatrische Onkologie, Hämatologie und Immunologie

MMPU

Start of MD project: tbd


Project description: 
Title:

Molecular Mechanisms underlying Hereditary Hemochromatosis
Summary:

The project aims to understand how a novel mutation that causes iron overload in a pediatric patient acts at the molecular level.
Methods that will be used:CRISPR/Cas Mutagenesis of cell lines, RNAi; mRNA and protein analyses
Reference:Muckenthaler MU, Rivella S, Hentze MW, Galy B: A Red Carpet for Iron Metabolism. Cell. 2017 Jan 26;168(3):344-361.
Cooperation partners:Matthias Hentze, European Molecular Biology Laboratory (EMBL); Martina Muckenthaler (University of Heidelberg)
Personal Qualifications:

Previous lab experience with molecular biology techniques

 

Keywords:Hemochromatosis, iron metabolism, hepcidin
Contact:

Andreas.Kulozik@med.uni-heidelberg.de

 

 

 

Project in Huber Group

Project leaderDr. Wolfgang Huber (EMBL) and Priv.Doz. Dr. Sascha Dietrich (Dept. of Internal Medicine V)
Project supervisor: Dr. Wolfgang Huber (EMBL) and Priv.Doz. Dr. Sascha Dietrich (Dept. of Internal Medicine V)
Affiliation:EMBL/MMPU
Start of MD project: tbd


Project description: 
Title:

Computational analysis and modelling of drug response heterogeneity in blood cancers based on large-scale ex-vivo assays and clinical data

Summary:

Therapy response in blood cancers can be highly variable. Possible reasons for therapy resistance comprise heterogeneous tumour genetics and variations in the role of the microenvironment. Our labs have established systems for large-scale ex-vivo testing of drug response across diverse tumours, large patient cohorts, and sophisticated co-culture systems that mimic the microenvironment in tumour niches.

You will take the responsibility of novel aspects in the bioinformatic and statist ical data analysis of these on-going studies, including high-throughput imaging of cells and single-cell RNA-seq, using machine learning and multivariate statistics methods. You will also get the opportunity to design and perform own follow-up experiments.

Referencehttps://www.jci.org/articles/view/93801
For more information please visit our website:

MMPU Group Systems Medicine of Cancer Drugs

Contact:

wolfgang.huber@embl.de

Sascha.Dietrich@embl.de

 

 

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