Kliniken & Institute … Institute Institute for… Research Sektion Molekulare… Publikationen

Publikationen

2021

Wabnitz GH, Honus S, Habicht J, Orlik C, Kirchgessner H, Samstag Y (2021). LFA-1 cluster formation in T-cells depends on L-plastin phosphorylation regulated by P90RSK and PP2A. Cell Mol Life Sci. 78(7):3543-3564. PubMed DOI

Balta E, Kramer J, Samstag Y (2021). Redox Regulation of the Actin Cytoskeleton in Cell Migration and Adhesion: On the Way to a Spatiotemporal View. Front Cell Dev Biol. 8:618261. PubMed DOI

2020

Samstag Y, Bogert NV, Wabnitz GH, Din S, Therre M, Leuschner F, Katus HA, Konstandin MH (2020). Reactive oxidative species-modulated Ca2+ release regulates β2 integrin activation on CD4+CD28null T cells of acute coronary syndrome patients. J Immunol. 205(8):2276-2286. PubMed DOI

Schönrich G, Raftery MJ, Samstag Y (2020). Devilishly radical NETwork in COVID-19: Oxidative stress, neutrophil extracellular traps (NETs), and T cell suppression. Adv Biol Regul. 77:100741. PubMed DOI

Wild B, Brenner J, Joos S, Samstag Y, Buckert M, Valentini J (2020). Acupuncture in persons with an increased stress level-Results from a randomized-controlled pilot trial. PLoS One. 15(7):e0236004. PubMed DOI

Liang J, Ziegler JD, Jahraus B, Orlik C, Blatnik R, Blank N, Niesler B, Wabnitz G, Ruppert T, Hübner K, Balta E, Samstag Y (2020). Piperlongumine Acts as an Immunosuppressant by Exerting Prooxidative Effects in Human T Cells Resulting in Diminished T H 17 but Enhanced T reg Differentiation. Front Immunol. 11:1172. PubMed DOI

Lederer AK, Maul-Pavicic A, Hannibal L, Hettich M, Steinborn C, Gründemann C, Zimmermann-Klemd AM, Müller A, Sehnert B, Salzer U, Klein R, Voll RE, Samstag Y, Huber R (2020). Vegan diet reduces neutrophils, monocytes and platelets related to branched-chain amino acids - A randomized, controlled trial. Clin Nutr. 39(11):3241-3250. PubMed DOI

Orlik C, Deibel D, Küblbeck J, Balta E, Ganskih S, Habicht J, Niesler B, Schröder-Braunstein J, Schäkel K, Wabnitz G, Samstag Y (2020). Keratinocytes costimulate naive human T cells via CD2: a potential target to prevent the development of proinflammatory Th1 cells in the skin. Cell Mol Immunol. 17(4):380-394. PubMed DOI

2019

Lederer AK, Hannibal L, Hettich M, Behringer S, Spiekerkoetter U, Steinborn C, Gründemann C, Zimmermann-Klemd AM, Müller A, Simmet T, Schmiech M, Maul-Pavicic A, Samstag Y, Huber R (2019). Vitamin B12 Status Upon Short-Term Intervention with a Vegan Diet-A Randomized Controlled Trial in Healthy Participants. Nutrients. 11(11):2815. PubMed DOI

Gülcüler Balta GS, Monzel C, Kleber S, Beaudouin J, Balta E, Kaindl T, Chen S, Gao L, Thiemann M, Wirtz CR, Samstag Y, Tanaka M, Martin-Villalba A (2019). 3D Cellular Architecture Modulates Tyrosine Kinase Activity, Thereby Switching CD95-Mediated Apoptosis to Survival. Cell Rep. 29(8):2295-2306. PubMed DOI

Cossarizza A, Chang HD, Radbruch A et al (2019). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol. 49(10):1457-1973. PubMed DOI

Balta E, Hardt R, Liang J, Kirchgessner H, Orlik C, Jahraus B, Hillmer S, Meuer S, Hübner K, Wabnitz GH, Samstag Y (2019). Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells. Nat Commun. 10(1):4073. PubMed DOI

Monaco S, Jahraus B, Samstag Y, Bading H (2019). Conditions of limited calcium influx (CLCI) inhibits IL2 induction and favors expression of anergy-related genes in TCR/CD3 and CD28 costimulated primary human T cells. Mol Immunol. 114:81-87 PubMed DOI

Schmitteckert S, Mederer T, Röth R, Günther P, Holland-Cunz S, Metzger M, Samstag Y, Schröder-Braunstein J, Wabnitz G, Kurzhals S, Scheuerer J, Beretta CA, Lasitschka F, Rappold GA, Romero P, Niesler B (2019). Postnatal human enteric neurospheres show a remarkable molecular complexity. Neurogastroenterol Motil. 31(10):e13674. PubMed DOI

Wu J, Ma S, Sandhoff R, Ming Y, Hotz-Wagenblatt A, Timmerman V, Bonello-Palot N, Schlotter-Weigel B, Auer-Grumbach M, Seeman P, Löscher WN, Reindl M, Weiss F, Mah E, Weisshaar N, Madi A, Mohr K, Schlimbach T, Velasco Cárdenas RM, Koeppel J, Grünschläger F, Müller L, Baumeister M, Brügger B, Schmitt M, Wabnitz G, Samstag Y, Cui G (2019). Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8(+) T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 50(5):1218-1231. PubMed DOI

Wortmann M, Xiao X, Wabnitz G, Samstag Y, Hakimi M, Böckler D, Dihlmann S (2019). AIM2 levels and DNA-triggered inflammasome response are increased in peripheral leukocytes of patients with abdominal aortic aneurysm. Inflamm Res. 68(4):337-345. PubMed DOI

Wabnitz G, Kirchgessner H, Samstag Y (2019). Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry. J Vis Exp. 143:e55345. PubMed DOI

Liang J, Hänsch GM, Hübner K, Samstag Y (2019). Sulforaphane as anticancer agent: A double-edged sword? Tricky balance between effects on tumor cells and immune cells. Adv Biol Regul. 71:79-87. Review. PubMed DOI

2018

Liang J, Jahraus B, Balta E, Ziegler JD, Hübner K, Blank N, Niesler B, Wabnitz GH, Samstag Y (2018). Sulforaphane Inhibits Inflammatory Responses of Primary Human T-Cells by Increasing ROS and Depleting Glutathione. Front Immunol. 9:2584. PubMed DOI

Wabnitz GH, Kirchgessner H, Jahraus B, Umansky L, Shenolikar S, Samstag Y (2018). Protein Phosphatase 1α and Cofilin Regulate Nuclear Translocation of NF-κB and Promote Expression of the Anti-Inflammatory Cytokine Interleukin-10 by T Cells. Mol Cell Biol. 38(22):e00041-18. PubMed DOI

Lacher SM, Thurm C, Distler U, Mohebiany AN, Israel N, Kitic M, Ebering A, Tang Y, Klein M, Wabnitz GH, Wanke F, Samstag Y, Bopp T, Kurschus FC, Simeoni L, Tenzer S, Waisman A (2018). NF-κB inducing kinase (NIK) is an essential post-transcriptional regulator of T-cell activation affecting F-actin dynamics and TCR signaling. J Autoimmun. 94:110-121. Pubmed DOI

Seeland I, Xiong Y, Orlik C, Deibel D, Prokosch S, Küblbeck G, Jahraus B, De Stefano D, Moos S, Kurschus FC, Arnold B, Samstag Y (2018). The actin remodeling protein cofilin is crucial for thymic αβ but not γδ T-cell development. PLoS Biol 16(7):e2005380. Pubmed DOI

2017

Klöss V, Grünvogel O, Wabnitz G, Eigenbrod T, Ehrhardt S, Lasitschka F, Lohmann V, Dalpke AH (2017). Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells. Front Immunol. 8:1238. PubMed DOI

Cossarizza A, Chang HD, Radbruch A, et al. (2017). Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol. 47(10):1584-1797.  PubMed DOI

Lasitschka F, Giese T, Paparella M, Kurzhals SR, Wabnitz G, Jacob K, Gras J, Bode KA, Heninger AK, Sziskzai T, Samstag Y, Leszinski C, Jocher B, Al-Saeedi M, Meuer SC, Schröder-Braunstein J (2017). Human monocytes downregulate innate response receptors following exposure to the microbial metabolite n-butyrate. Immun Inflamm Dis. 5(4):480-492. PubMed DOI

Sens C, Huck K, Pettera S, Uebel S, Wabnitz G, Moser M, Nakchbandi IA (2017). Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 292(19):7745-7760. PubMed DOI

Wabnitz GH, Kirchgessner H, Samstag Y (2017). Imaging Flow Cytometry for Multiparametric Analysis of Molecular Mechanism Involved in the Cytotoxicity of Human CD8(+) T-cells. J Cell Biochem. 118(9):2528-2533. PubMed DOI

2016

Monaco S, Jahraus B, Samstag Y, Bading H (2016). Nuclear calcium is required for human T cell activation. J Cell Biol. 215(2):231-243. PubMed DOI

Wabnitz G, Balta E, Samstag Y (2016). L-plastin regulates the stability of the immune synapse of naive and effector T-cells. Adv Biol Regul. 63:107-114. PubMed DOI

Wabnitz GH, Samstag Y (2016). Oxidative down-modulation of T cell-mediated cytotoxicity. Cell Death Dis. 7(9):e2373. PubMed DOI

Rossnagl S, Altrock E, Sens C, Kraft S, Rau K, Milsom MD, Giese T, Samstag Y, Nakchbandi IA (2016). EDA-Fibronectin Originating from Osteoblasts Inhibits the Immune Response against Cancer. PLoS Biol. 14(9): p. e1002562. PubMed DOI

Wabnitz GH, Balta E,  Schindler S, Kirchgessner H, Jahraus B, Meuer S, Samstag Y (2016). The pro-oxidative drug WF-10 inhibits serial killing by primary human cytotoxic T-cells. Cell Death Discov. 2:16057. PubMED DOI

Kraft S, Klemis V, Sens C, LenhardT, Jacobi C, Samstag Y, Wabnitz G, Kirschfink M, Wallich R, Hänsch GM,Nakchbandi IA (2016). Identification and characterization of a unique role for EDB fibronectin in phagocytosis.  J Mol Med (Berl). 94(5):567-81. PubMed DOI

Gaida MM, Mayer Y, Dapunt U, Stegmaier S, Schirmacher P, Wabnitz GH, Hänsch GM (2016). Expression of the bitter receptor T2R38 in pancreatic cancer: localization in lipid droplets and activation by a bacteria-derived quorum-sensing molecule. Oncotarget. 7(11):12623-32. PubMed DOI

Wabnitz GH, Samstag Y (2016). Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy. Methods Mol Biol. 1389:155-66. PubMed DOI

2015

Maurer S, Wabnitz GH, Kahle NA, Stegmaier S, Prior B, Giese T, Gaida MM, Samstag Y, Hänsch GM (2015). Tasting Pseudomonas aeruginosa biofilms: human neutrophils express the bitter receptor T2R38 as sensor for the quorum sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone. Front Immunol. 6 (369). PubMed DOI

Wabnitz GH, Nessmann A, Kirchgessner H, Samstag Y (2015). InFlow microscopy of human leukocytes: a tool for quantitative analysis of actin rearrangements in the immune synapse. J Immunol Methods. 423:29-39. PubMed DOI

2014

Schröder-Braunstein J, Gras J, Brors B, Schwarz S, Szikszai T, Lasitschka F, Wabnitz G, Heidtmann A, Lee YS, Schiessling S, Leowardi C, Al-Saeedi M, Ulrich A, Engelke A, Winter J, Samstag Y, Giese T, Meuer S (2014). Initiation of an inflammatory response in resident intestinal lamina propria cells –use of a human organ culture model. PLoS One. 9(5):e97780. PubMed DOI

Riplinger SM, Wabnitz GH, Kirchgessner H, Jahraus B, Lasitschka F, Schulte B, van der Pluijm G, van der Horst G, Hämmerling GJ, Nakchbandi I, Samstag Y (2014). Metastasis of prostate cancer and melanoma cells in a preclinical in vivo mouse model is enhanced by L-plastin expression and phosphorylation. Mol Cancer. 13:10. PubMed DOI

2013

Schulte B, John I, Simon B, Brockmann C, Oelmeier SA, Jahraus B, Kirchgessner H, Riplinger S, Carlomagno T, Wabnitz GH, Samstag Y (2013). A reducing milieu renders cofilin insensitive to Phosphatidylinositol 4,5-Bisphosphate (PIP2) inhibition. J Biol Chem. 288(41):29430-29439. PubMed DOI

Samstag Y, John I, Wabnitz GH (2013). Cofilin: a redox sensitive mediator of actin dynamics during T-cell activation and migration. Immunol Rev. 256:30-47. PubMed DOI

Munder M, Engelhardt M, Knies D, Medenhoff S, Wabnitz G, Luckner-Minden C, Feldmeyer N, Voss RH, Kropf P, Müller I, Conradi R, Samstag Y, Theobald M, Ho AD, Goldschmidt H, Hundemer M (2013). Cytotoxicity of tumor antigen specific human T cells is unimpaired by arginine depletion. PLoS One. 8(5):e63521. PubMed DOI

2012

Brechmann M, Mock T, Nickles D, Kiessling M, Weit N, Breuer R, Müller W, Wabnitz G, Frey F, Nicolay JP, Booken N, Samstag Y, Klemke CD, Herling M, Boutros M, Krammer PH, Arnold R (2012). A PP4 Holoenzyme Balances Physiological and Oncogenic Nuclear Factor-Kappa B Signaling in T Lymphocytes. Immunity. 37(4):697-708. PubMed DOI

Feldmeyer N, Wabnitz G, Leicht S, Luckner-Minden C, Schiller M, Franz T, Conradi R, Kropf P, Müller I, Ho AD, Samstag Y, Munder M (2012). Arginine deficiency leads to impaired cofilin dephosphorylation in activated human T lymphocytes. Int Immunol. 24(5):303-13. PubMed DOI

Autenrieth SE, Warnke P, Wabnitz GH, Lucero Estrada C, Pasquevich KA, Drechsler D, Günter M, Hochweller K, Novakovic A, Beer-Hammer S, Samstag Y, Hämmerling GJ, Garbi N, Autenrieth IB (2012). Depletion of dendritc cells enhances innate anti-bacterial host defense through modulation of phagocyte homeostasis. PLoS Pathog. 8(2):e1002552. PubMed DOI

2011

Wabnitz GH, Michalke F, Stober C, Kirchgessner H, Jahraus B, van den Boomen DJ, Samstag Y (2011). L-plastin phosphorylation: A novel target for the immunosuppressive drug dexamethasone in primary human T cells. Eur J Immunol. 41(11):3157-69. PubMed DOI

Kühne L, Konstandin M, Samstag Y, Meuer S, Giese T, Watzl C (2011). WF10 stimulates NK cell cytotoxicity by increasing LFA-1-mediated adhesion to tumor cells. J Biomed Biotechnol. 2011:436587. PubMed DOI

Hoffmann S, Hosseini BH, Hecker M, Louban I, Bulbuc N, Garbi N, Wabnitz GH, Samstag Y, Spatz JP, Hämmerling GJ (2011). Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells. Immunol Lett. 136(1):13-20. PubMed DOI

2010

Wabnitz GH, Lohneis P, Kirchgessner H, Jahraus B, Gottwald S, Konstandin M, Klemke M, Samstag Y (2010). Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin. Eur J Immunol. 40(9):2437-49. PubMed DOI

Klemke M, Kramer E, Konstandin MH, Wabnitz GH, Samstag Y (2010). An MEK-cofilin signalling module controls migration of human T cells in 3 D but not 2 D environments. EMBO J. 29(17):2915-29. PubMed DOI

Hoffmann SC, Wabnitz GH, Samstag Y, Moldenhauer G, Ludwig T (2010). Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lmphocyte and cancer cell interaction by single-cell force spectroscopy. Int J Cancer. 28(9):2096-2104. PubMed DOI

Hochweller K, Wabnitz GH, Samstag Y, Suffner J, Hämmerling GJ, Garbi N (2010). Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen. Proc Natl Acad Sci USA. 107(13):5931-5936. PubMed DOI

2009

Hosseini BH, Louban I, Djandji D, Wabnitz GH, Deeg J, Bulbuc N, Samstag Y, Gunzer M, Spatz JP, Hämmerling GJ (2009). Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy. Proc. Natl. Acad. Sci USA. 106(42):17852-17857. PubMed DOI

Gutscher M, Sobotta MC, Wabnitz GH, Ballikaya S, Meyer AJ, Samstag Y, Dick TP (2009). Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases. J Biol Chem. 284(46):31532-40. PubMed DOI

Konstandin MH, Aksoy H, Wabnitz GH, Volz C, Erbel C, Kirchgessner H, Giannitsis E, Katus HA, Samstag Y, Dengler TJ (2009). Beta2-integrin activation on T cell subsets is an independent prognostic factor in unstable angina pectoris. Basic Res Cardiol. 104(3):341-351. PubMed DOI

Günther F, Wabnitz GH, Stroh P, Prior B, Obst U, Samstag Y, Wagner C, Hänsch GM (2009). Host defence against Staphylococcus aureus biofilms infection: phagocytosis of biofilms by polymorphonuclear neutrophils (PMN). Mol. Immunol. 46(8-9):1805-1813. PubMed DOI

Klemke M, Samstag Y (2009). Molecular mechanisms mediating oxidative stress-induced T-cell suppression in cancer. Adv Enzyme Regul. 49(1):107-112. PubMed DOI

2008

Klemke M, Wabnitz GH, Funke F, Funk B, Kirchgessner H, Samstag Y (2008). Oxidation of cofilin mediates T cell hyporesponsiveness under oxidative stress conditions. Immunity. 29(3):404-413. PubMed DOI

Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, Meyer AJ, Dick TP (2008). Real-time imaging of the intracellular glutathione redox potential. Nat Methods. 5(6):553-559. PubMed DOI

2007

Braunstein J, Autschbach F, Giese T, Lasitschka F, Heidtmann A, Sido B, Funke B, Reiser C, Schröder AJ, Nebl G, Samstag Y, Meuer SC (2007). Up-regulation of the phosphoinositide 3-kinase pathway in lamina propria T lymphocytes. Clin Exp Immunol. 151(3):496-504. PubMed DOI

Wabnitz GH, Köcher T, Lohneis P, Stober C, Konstandin MH, Funk B, Sester U, Wilm M, Klemke M, Samstag Y (2007). Costimulation induced phosphorylation of L-plastin facilitates surface expression of the T cell activation markers CD69 and CD25. Eur J Immunol. 37(3):649-662. PubMed DOI

Klemke M, Weschenfelder T, Konstandin MH, Samstag Y (2007). High affinity interaction of integrin a4ß1 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) enhances migration of human melanoma cells across activated endothelial cell layers. J Cell Physiology212(2):368-374. PubMed DOI

Klemke M, Rafael MT, Wabnitz GH, Weschenfelder T, Konstandin MH, Garbi N, Autschbach F, Hartschuh W, Samstag Y (2007). Phosphorylation of ectopically expressed L-plastin enhances invasiveness of human melanoma cells. Int J Cancer. 120(12):2590-2599. PubMed DOI

Sester U, Wabnitz GH, Kirchgessner H, Samstag Y (2007). Ras/ PI3Kinase/cofilin independent activation of human CD45RA+ and CD45RO+ T-cells by superagonistic  CD28 stimulation. Eur J Immunol. 37(10):2881-2891. PubMed DOI

Konstandin MH, Wabnitz GH, Aksoy H, Kirchgessner H, Dengler TJ, Samstag Y (2007). A sensitive assay for the quantification of integrin-mediated adhesiveness of human stem cells and leukocyte subpopulations in whole blood. J Immunol Methods. 327(1-2):30-39. PubMed DOI

Samstag Y, M. Klemke (2007). Ectopic expression of L-plastin in human tumor cells: diagnostic and therapeutic implications. Adv Enzyme Regul. 47:118-126. PubMed DOI 

2006

Wabnitz GH, Nebl G, Klemke M, Schröder AJ, Samstag Y (2006). Phosphatidylinositol 3-kinase functions as a Ras effector in the signaling cascade that regulates dephosphorylation of the actin-remodeling protein cofilin after costimulation of untransformed human T lymphocytes. J Immunol. 176(3):1668-74. PubMed DOI

Konstandin MH, Sester U, Klemke M, Weschenfelder T, Wabnitz GH, Samstag Y (2006). A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins. J Immunol Methods. 310(1-2):67-77. PubMed DOI

2005

Samstag Y, Nebl G (2005). Ras initiates phosphatidyl-inositol-3-kinase (PI3K)/PKB mediated signalling pathways in untransformed human peripheral blood T lymphocytes. Adv Enzyme Regul. 45:52-62. PubMed DOI

2004

Nebl G, Fischer S, Penzel R, Samstag Y (2004). Dephosphorylation of cofilin is regulated through Ras and requires the combined activities of the Ras-effectors MEK and PI3K. Cell Signal. 16(2):235-243. PubMed DOI

Eibert SM, Lee KH, Pipkorn R, Sester U, Wabnitz GH, Giese T, Meuer SC, Samstag Y (2004). Cofilin peptide homologues interfere with immunological synapse formation and T cell activation. Proc Natl Acad Sci U S A. 101(7):1957-1962. PubMed DOI 

2003

Samstag Y, Nebl G (2003). Interaction of cofilin with the serine phosphatases PP1 and PP2A in normal and neoplastic human T lymphocytes. Adv Enzyme Regul. 43:197-211. PubMed DOI

Samstag Y, Eibert SM, Klemke M, Wabnitz GH (2003). Actin cytoskeletal dynamics in T lymphocyte activation and migration. J Leukoc Biol. 73(1):30-48. PubMed DOI

2000

Ambach A, Saunus J, Konstandin M, Wesselborg S, Meuer SC, Samstag Y (2000). The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes. Eur J Immunol. 30(12):3422-3431. PubMed DOI

Schröder AJ, Quehl P, Müller J, Samstag Y (2000). Conversion of p56(lck) to p60(lck) in human peripheral blood T lymphocytes is dependent on co- stimulation through accessory receptors: involvement of phospholipase C, protein kinase C and MAP-kinases in vivo. Eur J Immunol. 30(2):635-643. PubMed DOI       

Lee KH, Meuer SC, Samstag Y (2000). Cofilin: a missing link between T cell co-stimulation and rearrangement of the actin cytoskeleton. Eur J Immunol. 30(3):892-899. PubMed DOI