Kliniken & Institute … Institute Immunologie Forschung Sektion Molekulare… Publikationen

Publikationen (seit 2000)

Samstag Y, Bogert NV, Wabnitz GH, Din S, Therre M, Leuschner F, Katus HA, Konstandin MH (2020). Reactive oxidative species-modulated Ca2+ release regulates β2 integrin activation on CD4+CD28null T cells of acute coronary syndrome patients. J Immunol. Sep 16:ji2000327. PubMed DOI

Schönrich G, Raftery MJ, Samstag Y (2020). Devilishly radical NETwork in COVID-19: Oxidative stress, neutrophil extracellular traps (NETs), and T cell suppression. Adv Biol Regul. 77:100741. PubMed DOI

Wild B, Brenner J, Joos S, Samstag Y, Buckert M, Valentini J (2020). Acupuncture in persons with an increased stress level-Results from a randomized-controlled pilot trial. PLoS One. 15(7):e0236004. PubMed DOI

Liang J, Ziegler JD, Jahraus B, Orlik C, Blatnik R, Blank N, Niesler B, Wabnitz G, Ruppert T, Hübner K, Balta E, Samstag Y (2020). Piperlongumine Acts as an Immunosuppressant by Exerting Prooxidative Effects in Human T Cells Resulting in Diminished T H 17 but Enhanced T reg Differentiation. Front Immunol. 11:1172. PubMed DOI

Lederer AK, Maul-Pavicic A, Hannibal L, Hettich M, Steinborn C, Gründemann C, Zimmermann-Klemd AM, Müller A, Sehnert B, Salzer U, Klein R, Voll RE, Samstag Y, Huber R (2020). Vegan diet reduces neutrophils, monocytes and platelets related to
branched-chain amino acids - A randomized, controlled trial. Clin Nutr. Feb 24:S0261-5614(20)30059-5. PubMed DOI

Orlik C, Deibel D, Küblbeck J, Balta E, Ganskih S, Habicht J, Niesler B, Schröder-Braunstein J, Schäkel K, Wabnitz G, Samstag Y (2020). Keratinocytes costimulate naive human T cells via CD2: a potential target to prevent the development of proinflammatory Th1 cells in the skin. Cell Mol Immunol. 17(4):380-394. PubMed DOI

Lederer AK, Hannibal L, Hettich M, Behringer S, Spiekerkoetter U, Steinborn C, Gründemann C, Zimmermann-Klemd AM, Müller A, Simmet T, Schmiech M, Maul-Pavicic A, Samstag Y, Huber R (2019). Vitamin B12 status upon short-term intervention with a vegan diet-A randomized controlled trial in healthy participants. Nutrients. 11(11):2815.

Gülcüler Balta GS, Monzel C, Kleber S, Beaudouin J, Balta E, Kaindl T, Chen S, Gao L, Thiemann M, Wirtz CR, Samstag Y, Tanaka M, Martin-Villalba A (2019). 3D Cellular Architecture Modulates Tyrosine Kinase Activity, Thereby Switching CD95-Mediated Apoptosis to Survival. Cell Rep. 29(8):2295-2306.

Cossarizza A, Chang HD, Radbruch A et al (2019). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). Eur J Immunol. 49(10):1457-1973.

Balta E, Hardt R, Liang J, Kirchgessner H, Orlik C, Jahraus B, Hillmer S, Meuer S, Hübner K, Wabnitz GH, Samstag Y (2019). Spatial oxidation of L-plastin downmodulates actin-based functions of tumor cells. Nat Commun. 10(1):4073. PubMed DOI

Monaco S, Jahraus B, Samstag Y, Bading H (2019). Conditions of limited calcium influx (CLCI) inhibits IL2 induction and favors expression of anergy-related genes in TCR/CD3 and CD28 costimulated primary human T cells. Mol Immunol. 114:81-87 PubMed DOI

Schmitteckert S, Mederer T, Röth R, Günther P, Holland-Cunz S, Metzger M, Samstag Y, Schröder-Braunstein J, Wabnitz G, Kurzhals S, Scheuerer J, Beretta CA, Lasitschka F, Rappold GA, Romero P, Niesler B (2019). Postnatal human enteric neurospheres show a remarkable molecular complexity. Neurogastroenterol Motil. 31(10):e13674. PubMed DOI

Wu J, Ma S, Sandhoff R, Ming Y, Hotz-Wagenblatt A, Timmerman V, Bonello-Palot N, Schlotter-Weigel B, Auer-Grumbach M, Seeman P, Löscher WN, Reindl M, Weiss F, Mah E, Weisshaar N, Madi A, Mohr K, Schlimbach T, Velasco Cárdenas RM, Koeppel J, Grünschläger F, Müller L, Baumeister M, Brügger B, Schmitt M, Wabnitz G, Samstag Y, Cui G (2019). Loss of Neurological Disease HSAN-I-Associated Gene SPTLC2 Impairs CD8(+) T Cell Responses to Infection by Inhibiting T Cell Metabolic Fitness. Immunity. 50(5):1218-1231. PubMed DOI

Wortmann M, Xiao X, Wabnitz G, Samstag Y, Hakimi M, Böckler D, Dihlmann S (2019). AIM2 levels and DNA-triggered inflammasome response are increased in peripheral leukocytes of patients with abdominal aortic aneurysm. Inflamm Res. 68(4):337-345. PubMed DOI

Wabnitz G, Kirchgessner H, Samstag Y (2019). Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry. J Vis Exp. 143:e55345. PubMed DOI

Liang J, Hänsch GM, Hübner K, Samstag Y (2019). Sulforaphane as anticancer agent: A double-edged sword? Tricky balance between effects on tumor cells and immune cells. Adv Biol Regul. 71:79-87. Review. PubMed DOI

Liang J, Jahraus B, Balta E, Ziegler JD, Hübner K, Blank N, Niesler B, Wabnitz GH, Samstag Y (2018). Sulforaphane Inhibits Inflammatory Responses of Primary Human T-Cells by Increasing ROS and Depleting Glutathione. Front Immunol. 9:2584. PubMed DOI

Wabnitz GH, Kirchgessner H, Jahraus B, Umansky L, Shenolikar S, Samstag Y (2018). Protein Phosphatase 1α and Cofilin Regulate Nuclear Translocation of NF-κB and Promote Expression of the Anti-Inflammatory Cytokine Interleukin-10 by T Cells. Mol Cell Biol. 38(22):e00041-18. PubMed DOI

Lacher SM, Thurm C, Distler U, Mohebiany AN, Israel N, Kitic M, Ebering A, Tang Y, Klein M, Wabnitz GH, Wanke F, Samstag Y, Bopp T, Kurschus FC, Simeoni L, Tenzer S, Waisman A (2018). NF-κB inducing kinase (NIK) is an essential post-transcriptional regulator of T-cell activation affecting F-actin dynamics and TCR signaling. J Autoimmun pii:S0896-8411(18)30180-X. Pubmed DOI

Seeland I, Xiong Y, Orlik C, Deibel D, Prokosch S, Küblbeck G, Jahraus B, De Stefano D, Moos S, Kurschus FC, Arnold B, Samstag Y (2018). The actin remodeling protein cofilin is crucial for thymic αβ but not γδ T-cell development. PLoS Biol 16(7):e2005380. Pubmed DOI

Klöss V, Grünvogel O, Wabnitz G, Eigenbrod T, Ehrhardt S, Lasitschka F, Lohmann V, Dalpke AH (2017). Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells. Front Immunol. 8:1238. PubMed DOI

Cossarizza A, Chang HD, Radbruch A, et al. (2017). Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol. 47(10):1584-1797.  PubMed DOI

Lasitschka F, Giese T, Paparella M, Kurzhals SR, Wabnitz G, Jacob K, Gras J, Bode KA, Heninger AK, Sziskzai T, Samstag Y, Leszinski C, Jocher B, Al-Saeedi M, Meuer SC, Schröder-Braunstein J (2017). Human monocytes downregulate innate response receptors following exposure to the microbial metabolite n-butyrate. Immun Inflamm Dis. 6PubMed DOI

Sens C, Huck K, Pettera S, Uebel S, Wabnitz G, Moser M, Nakchbandi IA (2017). Fibronectins containing extradomain A or B enhance osteoblast differentiation via distinct integrins. J Biol Chem. 292(19):7745-7760. doi: 10.1074/jbc.M116.739987. PubMed PMID: 28325836; PubMed Central PMCID: PMC5427257.

Wabnitz GH, Kirchgessner H, Samstag Y (2017). Imaging Flow Cytometry for Multiparametric Analysis of Molecular Mechanism Involved in the Cytotoxicity of Human CD8(+) T-cells. J Cell Biochem. 118(9):2528-2533. doi: 10.1002/jcb.25963. Epub 2017 Apr 27. PubMed PMID: 28252214.

Monaco, S., B. Jahraus, Y. Samstag, H. Bading, 2016. Nuclear calcium is required for human T cell activation. J. Cell Biol. 215(2):231-243. Pubmed doi: 10.1083/jcb.201602001

Wabnitz, G.H., E. Balta, Y. Samstag, 2016. L-plastin regulates the stability of the immune synapse of naive and effector T-cells. Adv Biol Regul. pii: S2212-4926(16)30037-9. Pubmed doi: 10.1016/j.jbior.2016.09.009

Wabnitz, G.H., Y. Samstag, 2016. Oxidative down-modulation of T cell-mediated cytotoxicity. Cell Death Dis. 7(9):e2373. Pubmed doi: 10.1038/cddis.2016.273

Rossnagl, S., E. Altrock, C. Sens, S. Kraft, K. Rau, M. D. Milsom, T. Giese, Y. Samstag, I. A. Nakchbandi, 2016. EDA-Fibronectin Originating from Osteoblasts Inhibits the Immune Response against Cancer. PLoS Biol. 14(9): p. e1002562. Pubmed doi:10.1371/journal.pbio.1002562

Wabnitz, G.H., E. Balta, S. Schindler, H. Kirchgessner, B. Jahraus, S. Meuer, Y. Samstag, 2016. The pro-oxidative drug WF-10 inhibits serial killing by primary human cytotoxic T-cells. Cell Death Discov., 2:16057. Pubmed doi: 10.1038/cddiscovery.2016.57

Kraft, S., V. Klemis, C. Sens, T. Lenhard, C. Jacobi, Y. Samstag, G. Wabnitz, M. Kirschfink, R. Wallich, G.M. Hänsch, I.A. Nakchbandi, 2016. Identification and characterization of a unique role for EDB fibronectin in phagocytosis.  J Mol Med. (Berl) 94(5):567-81. Pubmed doi: 10.1007/s00109-015-1373-0

Gaida, M.M., C. Mayer, U. Dapunt, S. Stegmaier, P. Schirmacher, G.H. Wabnitz, G.M. Hänsch, 2016. Expression of the bitter receptor T2R38 in pancreatic cancer: localization in lipid droplets and activation by a bacteria-derived quorum-sensing molecule. Oncotarget. 7(11):12623-32. Pubmed doi: 10.18632/oncotarget.7206

Wabnitz, G.H., Y. Samstag, 2016. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy. Methods Mol Biol. 1389:155-66. doi: 10.1007/978-1-4939-3302-0_10.

Maurer, S., G.H. Wabnitz, N.A. Kahle, S. Stegmaier, B. Prior, T. Giese, M.M. Gaida, Y. Samstag, G.M. Hänsch, 2015. Tasting Pseudomonas aeruginosa biofilms: human neutrophils express the bitter receptor T2R38 as sensor for the quorum sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone. Front Immunol., 6 (369). doi: 10.3389/fimmu.2015.00369.

Wabnitz, G.H., A. Nessmann, H. Kirchgessner, Y. Samstag, 2015. InFlow microscopy of human leukocytes: a tool for quantitative analysis of actin rearrangements in the immune synapse. J Immunol Methods. 423: 29-39. doi: 10.1016/j.jim.2015.03.003.

Schröder-Braunstein, J., J. Gras, B. Brors, S. Schwarz, T. Szikszai, F. Lasitschka, G. Wabnitz, A. Heidtmann, YS. Lee, S. Schiessling, C. Leowardi, M. Al-Saeedi, A. Ulrich, A. Engelke, J. Winter, Y. Samstag, T. Giese, S. Meuer, 2014. Initiation of an inflammatory response in resident intestinal lamina propria cells –use of a human organ culture model. PLoS One, 9(5): e97780. doi: 10.1371/journal.pone.0097780.

Riplinger, S.M., G.H. Wabnitz, H. Kirchgessner, B. Jahraus, F. Lasitschka, B. Schulte, G. van der Pluijm, G.van der Horst, G.J. Hämmerling, I. Nakchbandi, Y. Samstag, 2014. Metastasis of prostate cancer and melanoma cells in a preclinical in vivo mouse model is enhanced by L-plastin expression and phosphorylation. Mol. Cancer, 13(1):10.

Schulte, B., I. John, B. Simon, Ch. Brockmann, S.A. Oelmeier, B. Jahraus, H. Kirchgessner, S. Riplinger, T. Carlomagno, G.H. Wabnitz, and Y. Samstag, 2013. A reducing milieu renders cofilin insensitive to Phosphatidylinositol 4,5-Bisphosphate (PIP2) inhibition. J Biol Chem. 288(41):29430-29439.

Samstag, Y., I. John, and G.H. Wabnitz, 2013. Cofilin: a redox sensitive mediator of actin dynamics during T-cell activation and migration. Immunol Rev. 256:30-47.

Munder, M., M. Engelhardt, D. Knies, S. Medenhoff, G.H. Wabnitz, C. Luckner-Minden, N. Feldmeyer, R.H. Voss, P. Kropf, I. Müller, R. Conradi, Y. Samstag, M. Theobald, A.D. Ho, H. Goldschmidt, and M. Hundemer, 2013. Cytotoxicity of tumor antigen specific human T cells is unimpaired by arginine depletion. PLoS One, 8 (5):e63521. doi:10.1371/journal.pone.0063521

Brechmann, M., T. Mock, D. Nickles, M. Kiessling,  N. Weit, R. Breuer, W. Müller, G. Wabnitz, F. Frey, J.P. Nicolay, N. Booken, Y. Samstag, C.D. Klemke, M. Herling, M. Boutros, P.H. Krammer, and R. Arnold, 2012. A PP4 Holoenzyme Balances Physiological and Oncogenic Nuclear Factor-Kappa B Signaling in T Lymphocytes. Immunity, 37 (4): 697-708.

Feldmeyer, N., G. H. Wabnitz, S. Leicht, C. Luckner-Minden, M. Schiller, T. Franz, R. Conradi, P. Kropf, I. Müller, A.D. Ho, Y. Samstag, and M. Munder, 2012. Arginine deficiency leads to impaired cofilin dephosphorylation in activated human T lymphocytes. International Immunology, 24 (5): 303-13.

Autenrieth, S.E., P. Warnke, G.H. Wabnitz, C. L. Estrada, K.A. Pasquevich, D. Drechsler, M. Günter, K. Hochweller, A. Novakovic, Y. Samstag, G. J. Hämmerling, N. Garbi, and I. B. Autenrieth, 2012. Depletion of dendritc cells enhances innate anti-bacterial host defense through modulation of phagocyte homeostasis. PLoS Pathogens, 8 (2): e1002552.

Wabnitz, G.H., F. Michalke, C. Stober, H. Kirchgessner, B. Jahraus, D.J. van den Boomen, and Y. Samstag, 2011. L-plastin phosphorylation: A novel target for the immunosuppressive drug dexamethasone in primary human T cells. Eur. J. Immunol. 41 (11):3157-69.

Kühne, L., M. Konstandin, Y. Samstag, S. Meuer, T. Giese, and C. Watzl, 2011. WF10 stimulates NK cell cytotoxicity by increasing LFA-1-mediated adhesion to tumor cells. Journal of Biomedicine and Biotechnology. 2011:436587. Epub.

Hoffmann, S., B.H. Hosseini, M. Hecker, I. Louban, N. Bulbuc, N. Garbi, G.H. Wabnitz, Y. Samstag, J.P. Spatz, and G.J. Hämmerling, 2011. Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells. Immunol. Lett. 136 (1): 13-20.

Wabnitz, G.H., Lohneis, P., Kirchgessner, H., Jahraus, B., Gottwald, S., Konstandin, M., Klemke M., Y. Samstag, 2010. Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin. Eur. J. Immunol. 40 (9): 2437-49.

Klemke, M., Kramer, E., Konstandin, M.H., Wabnitz, G.H., Y. Samstag, 2010. An MEK-cofilin signalling module controls migration of human T cells in 3 D but not 2 D environments. EMBO J. 29(17): 2915-29.

Hoffmann, S.C., Wabnitz, G.H., Y. Samstag, Moldenhauer, G., Ludwig, T., 2010. Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lmphocyte and cancer cell interaction by single-cell force spectroscopy. Int. J. Cancer., {Epub ahead of print}

Hochweller, K., Wabnitz G.H., Y. Samstag, Suffner, J., Hämmerling, G.J., Garbi N., 2010. Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen. Proc. Natl. Acad. Sci. USA 107 (13): 5931-6.

Hosseini, B.H., Louban, I. Djandji, D., Wabnitz, G.H., Deeg, J., Bulbuc N., Y. Samstag, Gunzer M., Spatz J.P., Hämmerling G.J., 2009. Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy. Proc. Natl. Acad. Sci USA 106 (42): 17852-7.

Gutscher, M., Sobotta MC., Wabnitz, GH., Ballikaya, S., Meyer AJ., Y. Samstag., Dick TP., 2009. Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases. J. Biol. Chem. 284 (46): 31532-40.

Konstandin, M.H., Aksoy, H., Wabnitz, G.H., Volz, C., Erbel C., Kirchgessner H., Giannitsis, E., Katus, H.A., Y. Samstag, Dengler, T.J., 2009. Beta2-integrin activation on T cell  subsets is an independent prognostic factor in unstable angina pectoris. Basic Res. Cardiol. 104 (3): 341-51.

Günther, F., Wabnitz, G.H., Stroh, P., Prior B., Obst, U., Y. Samstag, Wagner, C., Hänsch G.M., 2009. Host defence against Staphylococcus aureus biofilms infection: phagocytosis of biofilms by polymorphonuclear neutrophils (PMN). Mol. Immunol. 46 (8-9): 1805-13.

Klemke, M., Y. Samstag, 2009. Molecular mechanisms mediating oxidative stress-induced T-cell suppression in cancer. Adv. Enzyme Regul. 49 (1): 107-12.

Klemke, M., G.H. Wabnitz, F. Funke, B. Funk, H. Kirchgessner, and Y. Samstag, 2008. Oxidation of cofilin mediates T cell hyporesponsiveness under oxidative stress conditions. Immunity 29: 404-413.

Gutscher, M., A.-L. Pauleau, L. Marty, T. Brach, G.H. Wabnitz, Y. Samstag, A.J. Meyer, and T.P. Dick, 2008. Real-time imaging of the intracellular glutathione redox potential. Nature Methods 5 (6): 553-559.

Braunstein, J., F. Autschbach, T. Giese, F. Lasitschka, A. Heidtmann, B. Sido, B. Funke, C. Reiser, A. J. Schröder, G. Nebl, Y. Samstag, and S. Meuer, 2007. Up-regulation of the phosphoinositide 3-kinase pathway in lamina propria T lymphocytes. Clin. Exp. Immunol. 151 (3): 496-504.

Wabnitz, G., T. Köcher, C. Stober, M. Konstandin, B. Funk, M. Wilm, U. Sester, M. Klemke, and Y. Samstag, 2007. Costimulation induced phosphorylation of L-plastin facilitates surface expression of the T cell activation markers CD69 and CD25. Eur. J. Immunol.  37: 649-662.

Klemke, M., T. Weschenfelder, M. H. Konstandin, and Y. Samstag 2007. High affinity interaction of integrin a4ß1 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) enhances migration of human melanoma cells across activated endothelial cell layers. J. Cell. Physiology  21: 368-347.

Klemke, M., M.T. Rafael, G. Wabnitz, T. Weschenfelder, M. H. Konstandin, F. Autschbach, W. Hartschuh, and Y. Samstag 2007. Phosphorylation of ectopically expressed L-plastin enhances invasiveness of human melanoma cells. Int. J. Cancer 120: 2590-2599.

Sester, U., G. Wabnitz, H. Kirchgessner, and Y. Samstag 2007. Ras/ PI3Kinase/cofilin independent activation of human CD45RA+ and CD45RO+ T-cells by superagonistic  CD28 stimulation. Eur. J. Immunol. 37: 2881-2891.

Konstandin, M. H., U. Sester, M. Klemke, T. Weschenfelder, G. H. Wabnitz, and Y. Samstag 2007. A sensitive assay for the quantification of integrin-mediated adhesiveness of human stem cells and leukocyte subpopulations in whole blood. J. Immunol. Methods 327: 30-39.

Samstag, Y., and M. Klemke 2007. Ectopic expression of L-plastin in human tumor cells: diagnostic and therapeutic implications. Advances in Enzyme Regulation 47: 118-126

Wabnitz, G. H., G. Nebl, M. Klemke, A. J. Schroeder, and Y. Samstag 2006. Phosphatidylinositol 3-kinase  functions as Ras effector in the signalling cascade that regulates dephosphorylation  of the actin-remodeling  protein cofilin after costimulation of untransformed human T lymphocytes. J. Immunol. 176: 1668-1674.

Konstandin, M., U. Sester, M. Klemke, T. Weschenfelder, G. H. Wabnitz, and Y. Samstag 2006. A novel flow-cytometry based assay for quantification of affinity and avidity changes of integrins. J. Immunol. Methods 310: 67-77.

Samstag, Y., and G. Nebl 2005. Ras initiates phosphatidyl-inositol-3-kinase (PI3K)/PKB mediated signalling pathways in untransformed human peripheral blood T lymphocytes. Advances in Enzyme Regulation 45: 52-62.

Nebl, G., S. Fischer, R. Penzel, and Y. Samstag 2004. Dephosphorylation of Cofilin is regulated through Ras and requires the combined activites of the Ras-effectors  PI3K/PKB and MEK. Cell. Signalling 16: 235-243.

Eibert, S., K. H. Lee, W. Pipkorn, U. Sester, G.H. Wabnitz, T. Giese, S. C. Meuer, and Y. Samstag 2004. Cofilin peptide homologues interfere with immunological synapse formation and T cell activation. Proc. Natl. Acad. Sci. USA (PNAS) 10: 1957–1962.

Samstag, Y., and G. Nebl 2003. Interaction of cofilin with the serine phosphatases PP1 and PP2A in normal and neoplastic human T lymphocytes. Advances in Enzyme Regulation 43: 197-211.

Samstag, Y.,  S.M. Eibert, M. Klemke, and G.H. Wabnitz 2003. Actin cytoskeletal dynamics in T cell activation and migration. J. Leukocyte Biology 73: 30-48.

Ambach, A., J. Saunus, M. Konstandin, S. Wesselborg, S. C. Meuer, and Y. Samstag 2000. The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes. Eur. J. Immunol. 30: 3422-3431. 

Schroeder, A.J., P. Quehl, J. Müller, and Y. Samstag 2000. Conversion of p56lck to p60lck in human peripheral blood T lymphocytes is dependent on costimulation through accessory receptors: involve­ment of phospholipase C, protein kinase C and MAP-kinases in vivo. Eur. J. Immunol. 30: 635-643.        

Lee, K.-H., S.C. Meuer and Y. Samstag 2000. Cofilin: A missing link between T cell costimulation and rearrangement of the actin cytoskeleton. Eur. J. Immunol.  30: 892-899.