We offer support for a range of phenotyping techniques to characterize iPSC-derived cells and 3D tissue models at molecular, cellular, and functional levels. Functional assays such as calcium imaging, electrophysiology, and contractility measurements provide detailed insights into cellular performance. In addition, high-content imaging and automated data analysis ensure quantitative and reproducible results. Together, these methods enable comprehensive phenotypic profiling for quality control, disease modeling, and drug testing.
Cardiomyocyte Contractility Assay
Cardiomyocyte contractility assays measure the beating behavior and contractile strength of heart muscle cells derived from iPSCs. They provide functional insights into cardiac performance, drug responses, and disease-related alterations in contractile activity.
Fura-2 calcium measurement is a fluorescence-based assay used to monitor intracellular calcium levels in living cells. By detecting changes in Fura-2 emission ratios, it enables precise analysis of calcium dynamics during cellular signaling and excitation–contraction processes.
EHT force measurement with PDMS posts allows quantification of contractile strength by tracking the deflection of flexible posts during tissue contraction. This method provides a direct and reproducible assessment of contractile force under physiological or experimental conditions.
Microtissues Contractility Assay
Microtissue contractility assays evaluate the mechanical activity of microtissues by measuring their deformation or force generation during contraction. They enable quantitative analysis of tissue functionality and responses to pharmacological or environmental stimuli.
