Reprogramming

Reprogramming refers to the conversion of somatic cells, such as skin fibroblasts or blood cells, into induced pluripotent stem cells (iPSCs). This process is achieved by introducing defined transcription factors that reset the cells into a pluripotent state. The generated iPSCs can then be expanded indefinitely and differentiated into various cell types, providing a renewable source of patient-specific material for research and applications.

Genome Editing

Genome editing allows targeted changes to the DNA of stem cells. With tools such as CRISPR/Cas, specific genes can be knocked out, inserted, or corrected, enabling the creation of customized cellular models. Edited iPSCs can be used to study disease mechanisms, validate genetic findings, or establish isogenic control lines, thereby providing powerful tools for basic and translational research.

iPSC derived cell types

iPSC-CMs (Cardiomyocytes)
iPSC-derived cardiomyocytes represent the beating muscle cells of the heart. They are widely used to study cardiac physiology, model inherited heart diseases, and test the effects of drugs on contractile and electrophysiological function.

iPSC-CFs (Cardiac Fibroblasts)
iPSC-derived cardiac fibroblasts form the supportive matrix of the heart and regulate tissue remodeling. They are useful for studying fibrosis, extracellular matrix production, and interactions with cardiomyocytes in healthy and diseased conditions.

iPSC-ECs (Endothelial Cells)
iPSC-derived endothelial cells form the inner lining of blood vessels. They provide a model to investigate angiogenesis, vascular barrier function, and inflammatory processes relevant to cardiovascular and systemic diseases.

3D models

Engineered heart tissue (EHT)

Engineered heart tissues (EHTs) are three-dimensional cardiac muscle models generated from iPSC-CMs. They mimic key structural and functional properties of the human heart and are used to study cardiac physiology, disease, and

Microtissues

 

Microtissues are small, three-dimensional cell aggregates that replicate essential features of native tissue organization and function. They provide physiologically relevant models for studying cell interactions, tissue development, and drug effects in vitro.

Quality Control

Quality control ensures that stem cell lines are reliable and safe to use in downstream experiments. It involves testing for pluripotency markers, verifying genomic stability, checking for mycoplasma or other contaminations, and confirming correct identity of the lines. These measures help to maintain reproducibility across projects and ensure that iPSCs remain suitable for differentiation and functional studies.