Immunologie

Sektion Molekulare Immunologie

 

Yvonne Samstag, Prof. Dr. med.

W3-Professorin für Zelluläre Immunologie

e-mail: yvonne.samstag@urz.uni-heidelberg.de

Tel.: +49-6221-56-4039, FAX +49-6221-56-5549

 

Mitarbeiter:

Guido Wabnitz, Dr. rer. nat. (Dipl. Biol.)

Selina Riplinger, Dr. rer. nat. (Dipl. Biol.)

Beate Jahraus (Dipl. Biol., Technische Assistentin)

Henning Kirchgessner (Technischer Angestellter)

Bianca Schulte (Dipl. Biol., Doktorandin)

Daniel Deibel (Dipl. Biol., Doktorand)

Isabel John (M.Sc., Doktorandin)

Sybille Honus, Cand. med.

Irmgard Barnes, Sekretärin

 

Offene Stellen:

 

 

 Forschungsprojekte

 

Kostimulatorische Signale der T-Zellaktivierung als Angriffspunkte für die therapeutische Induktion bzw. Durchbrechung von Toleranz

 

PfeilBedeutung der ektopen Expression von L-Plastin für die Migration, Invasion und Metastasierung von Tumorzellen – oder: wie Krebszellen von Immunzellen das Wandern lernen

 

PfeilAktivierung des spezifischen zellulären Immunsystems bei akutem Koronarsyndrom

 

 




 

 

 

Publikationen (seit 2000)

 

 

 

Munder, M., M. Engelhardt, D. Knies, S. Medenhoff, G.H. Wabnitz, C. Luckner-Minden, N. Feldmeyer, R.H. Voss, P. Kropf, I. Müller, R. Conradi, Y. Samstag, M. Theobald, A.D. Ho, H. Goldschmidt, and M. Hundemer, 2013. Cytotoxicity of tumor antigen specific human T cells is unimpaired by arginine depletion. PLoS One, 8 (5): e63521. Doi:10.1371/journal.pone.0063521 (23 May 2013)

 

Brechmann, M., T. Mock, D. Nickles, M. Kiessling,  N. Weit, R. Breuer, W. Müller, G. Wabnitz, F. Frey, J.P. Nicolay, N. Booken, Y. Samstag, C.D. Klemke, M. Herling, M. Boutros, P.H. Krammer, and R. Arnold, 2012. A PP4 Holoenzyme Balances Physiological and Oncogenic Nuclear Factor-Kappa B Signaling in T Lymphocytes. Immunity, 37 (4): 697-708.

 

Feldmeyer, N., G. H. Wabnitz, S. Leicht, C. Luckner-Minden, M. Schiller, T. Franz, R. Conradi, P. Kropf, I. Müller, A.D. Ho, Y. Samstag, and M. Munder, 2012. Arginine deficiency leads to impaired cofilin dephosphorylation in activated human T lymphocytes. International Immunology, 24 (5): 303-13.

 

Autenrieth, S.E., P. Warnke, G.H. Wabnitz, C. L. Estrada, K.A. Pasquevich, D. Drechsler, M. Günter, K. Hochweller, A. Novakovic, Y. Samstag, G. J. Hämmerling, N. Garbi, and I. B. Autenrieth, 2012. Depletion of dendritc cells enhances innate anti-bacterial host defense through modulation of phagocyte homeostasis. PLoS Pathogens, 8 (2): e1002552.

 

Wabnitz, G.H., F. Michalke, C. Stober, H. Kirchgessner, B. Jahraus, D.J. van den Boomen, and Y. Samstag, 2011. L-plastin phosphorylation: A novel target for the immunosuppressive drug dexamethasone in primary human T cells. Eur. J. Immunol. 41 (11):3157-69.

 

Kühne, L., M. Konstandin, Y. Samstag, S. Meuer, T. Giese, and C. Watzl, 2011. WF10 stimulates NK cell cytotoxicity by increasing LFA-1-mediated adhesion to tumor cells. Journal of Biomedicine and Biotechnology. 2011:436587. Epub.

 

Hoffmann, S., B.H. Hosseini, M. Hecker, I. Louban, N. Bulbuc, N. Garbi, G.H. Wabnitz, Y. Samstag, J.P. Spatz, and G.J. Hämmerling, 2011. Single cell force spectroscopy of T cells recognizing a myelin-derived peptide on antigen presenting cells. Immunol. Lett. 136 (1): 13-20.

 

Wabnitz, G.H., Lohneis, P., Kirchgessner, H., Jahraus, B., Gottwald, S., Konstandin, M., Klemke M., Y. Samstag, 2010. Sustained LFA-1 cluster formation in the immune synapse requires the combined activities of L-plastin and calmodulin. Eur. J. Immunol. 40 (9): 2437-49.

 

Klemke, M., Kramer, E., Konstandin, M.H., Wabnitz, G.H., Y. Samstag, 2010. An MEK-cofilin signalling module controls migration of human T cells in 3 D but not 2 D environments. EMBO J. 29(17): 2915-29.

 

Hoffmann, S.C., Wabnitz, G.H., Y. Samstag, Moldenhauer, G., Ludwig, T., 2010. Functional analysis of bispecific antibody (EpCAMxCD3)-mediated T-lmphocyte and cancer cell interaction by single-cell force spectroscopy. Int. J. Cancer., {Epub ahead of print}

 

Hochweller, K., Wabnitz G.H., Y. Samstag, Suffner, J., Hämmerling, G.J., Garbi N., 2010. Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen. Proc. Natl. Acad. Sci. USA 107 (13): 5931-6.

 

Hosseini, B.H., Louban, I. Djandji, D., Wabnitz, G.H., Deeg, J., Bulbuc N., Y. Samstag, Gunzer M., Spatz J.P., Hämmerling G.J., 2009. Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy. Proc. Natl. Acad. Sci USA 106 (42): 17852-7.

 

Gutscher, M., Sobotta MC., Wabnitz, GH., Ballikaya, S., Meyer AJ., Y. Samstag., Dick TP., 2009. Proximity-based protein thiol oxidation by H2O2-scavenging peroxidases. J. Biol. Chem. 284 (46): 31532-40.

 

Konstandin, M.H., Aksoy, H., Wabnitz, G.H., Volz, C., Erbel C., Kirchgessner H., Giannitsis, E., Katus, H.A., Y. Samstag, Dengler, T.J., 2009. Beta2-integrin activation on T cell  subsets is an independent prognostic factor in unstable angina pectoris. Basic Res. Cardiol. 104 (3): 341-51.

 

Günther, F., Wabnitz, G.H., Stroh, P., Prior B., Obst, U., Y. Samstag, Wagner, C., Hänsch G.M., 2009. Host defence against Staphylococcus aureus biofilms infection: phagocytosis of biofilms by polymorphonuclear neutrophils (PMN). Mol. Immunol. 46 (8-9): 1805-13.

 

Klemke, M., Y. Samstag, 2009. Molecular mechanisms mediating oxidative stress-induced T-cell suppression in cancer. Adv. Enzyme Regul. 49 (1): 107-12.

 

Klemke, M., G.H. Wabnitz, F. Funke, B. Funk, H. Kirchgessner, and Y. Samstag, 2008. Oxidation of cofilin mediates T cell hyporesponsiveness under oxidative stress conditions. Immunity 29: 404-413.

 

Gutscher, M., A.-L. Pauleau, L. Marty, T. Brach, G.H. Wabnitz, Y. Samstag, A.J. Meyer, and T.P. Dick, 2008. Real-time imaging of the intracellular glutathione redox potential. Nature Methods 5 (6): 553-559.

 

Braunstein, J., F. Autschbach, T. Giese, F. Lasitschka, A. Heidtmann, B. Sido, B. Funke, C. Reiser, A. J. Schröder, G. Nebl, Y. Samstag, and S. Meuer, 2007. Up-regulation of the phosphoinositide 3-kinase pathway in lamina propria T lymphocytes. Clin. Exp. Immunol. 151 (3): 496-504.

 

Wabnitz, G., T. Köcher, C. Stober, M. Konstandin, B. Funk, M. Wilm, U. Sester, M. Klemke, and Y. Samstag, 2007. Costimulation induced phosphorylation of L-plastin facilitates surface expression of the T cell activation markers CD69 and CD25. Eur. J. Immunol.  37: 649-662.

 

Klemke, M., T. Weschenfelder, M. H. Konstandin, and Y. Samstag 2007. High affinity interaction of integrin a4ß1 (VLA-4) and vascular cell adhesion molecule 1 (VCAM-1) enhances migration of human melanoma cells across activated endothelial cell layers. J. Cell. Physiology  21: 368-347.

 

Klemke, M., M.T. Rafael, G. Wabnitz, T. Weschenfelder, M. H. Konstandin, F. Autschbach, W. Hartschuh, and Y. Samstag 2007. Phosphorylation of ectopically expressed L-plastin enhances invasiveness of human melanoma cells. Int. J. Cancer 120: 2590-2599.

 

Sester, U., G. Wabnitz, H. Kirchgessner, and Y. Samstag 2007. Ras/ PI3Kinase/cofilin independent activation of human CD45RA+ and CD45RO+ T-cells by superagonistic  CD28 stimulation. Eur. J. Immunol. 37: 2881-2891.

 

Konstandin, M. H., U. Sester, M. Klemke, T. Weschenfelder, G. H. Wabnitz, and Y. Samstag 2007. A sensitive assay for the quantification of integrin-mediated adhesiveness of human stem cells and leukocyte subpopulations in whole blood. J. Immunol. Methods 327: 30-39.

 

Samstag, Y., and M. Klemke 2007. Ectopic expression of L-plastin in human tumor cells: diagnostic and therapeutic implications. Advances in Enzyme Regulation 47: 118-126

 

Wabnitz, G. H., G. Nebl, M. Klemke, A. J. Schroeder, and Y. Samstag 2006. Phosphatidylinositol 3-kinase  functions as Ras effector in the signalling cascade that regulates dephosphorylation  of the actin-remodeling  protein cofilin after costimulation of untransformed human T lymphocytes. J. Immunol. 176: 1668-1674.

 

Konstandin, M., U. Sester, M. Klemke, T. Weschenfelder, G. H. Wabnitz, and Y. Samstag 2006. A novel flow-cytometry based assay for quantification of affinity and avidity changes of integrins. J. Immunol. Methods 310: 67-77.

 

Samstag, Y., and G. Nebl 2005. Ras initiates phosphatidyl-inositol-3-kinase (PI3K)/PKB mediated signalling pathways in untransformed human peripheral blood T lymphocytes. Advances in Enzyme Regulation 45: 52-62.

 

Nebl, G., S. Fischer, R. Penzel, and Y. Samstag 2004. Dephosphorylation of Cofilin is regulated through Ras and requires the combined activites of the Ras-effectors  PI3K/PKB and MEK. Cell. Signalling 16: 235-243.

 

Eibert, S., K. H. Lee, W. Pipkorn, U. Sester, G.H. Wabnitz, T. Giese, S. C. Meuer, and Y. Samstag 2004. Cofilin peptide homologues interfere with immunological synapse formation and T cell activation. Proc. Natl. Acad. Sci. USA (PNAS) 10: 1957–1962.

 

Samstag, Y., and G. Nebl 2003. Interaction of cofilin with the serine phosphatases PP1 and PP2A in normal and neoplastic human T lymphocytes. Advances in Enzyme Regulation 43: 197-211.

 

Samstag, Y.,  S.M. Eibert, M. Klemke, and G.H. Wabnitz 2003. Actin cytoskeletal dynamics in T cell activation and migration. J. Leukocyte Biology 73: 30-48.

 

Ambach, A., J. Saunus, M. Konstandin, S. Wesselborg, S. C. Meuer, and Y. Samstag 2000. The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes. Eur. J. Immunol. 30: 3422-3431. 

 

Schroeder, A.J., P. Quehl, J. Müller, and Y. Samstag 2000. Conversion of p56lck to p60lck in human peripheral blood T lymphocytes is dependent on costimulation through accessory receptors: involve­ment of phospholipase C, protein kinase C and MAP-kinases in vivo. Eur. J. Immunol. 30: 635-643.        

 

Lee, K.-H., S.C. Meuer and Y. Samstag 2000. Cofilin: A missing link between T cell costimulation and rearrangement of the actin cytoskeleton. Eur. J. Immunol.  30: 892-899.

Für einen vollständigen Publikationsindex nutzen Sie bitte die
Pfeil Externer LinkSuche auf PubMed.

 

 

Abgeschlossene Dissertationen (seit 2000)

 

Selina Riplinger 2012 (Dr. rer.nat.):

Phosphoryliertes L-Plastin verstärkt die Tumorzellmetastasierung

Felix Michalke 2011 (Dr. med.):

Einfluss von Immunsuppressiva auf die Entstehung und Reifung der immunologischen Synapse

Catherine Goursot 2011 (Dr. med.):

Role of cofilin in the programmed cell death induced by oxidative stress in human T lymphocytes

Philipp Lohneis 2009 (Dr. med.):

L-Plastin in humanen T-Lymphozyten: Calmodulin-abhängige Verlagerung an die immunologische Synapse.

Christoph Stober 2006 (Dr. med.):

L-Plastin, ein neues Zielprotein der immunsuppressiven Therapie.

Maria Rafael 2005 (Dr. med.):

Immunhistologische Untersuchung der Expression des Aktin-bündelnden Proteins L-Plastin in nicht-hämatopoetischen humanen Tumoren: Vergleich mit Tumorstadium und Metastasierungsstatus.

Guido Wabnitz 2004 (Dr. rer.nat.):

L-Plastin in humanen T-Lymphozyten: Kostimulations-abhängige Phosphorylierung an Ser-5 und Verlagerung in die immunologische Synapse.

Sybille Eibert 2003 (Dr. rer. nat.):

Cofilin/Aktin-Wechselwirkungen sind notwendig für die Bildung der immunologischen Synapse und für die T-Zellaktivierung.

Mathias Konstandin 2001 (Dr. med.):

Einfluss von Immunsuppressiva auf kostimulatorische Signale der T-Zellaktivierung: Cofilin-Dephosphorylierung und Lck-Konversion

Eva Dreizler 2000 (Dr. rer. nat.):           

Translokation von Aktin / Cofilin-Komplexen in den Zellkern: ein anti-apoptotischer Mechanismus?

 

 

Select languageSelect language
Print Mail