Kliniken & Institute … Institute Zentrum für… Parasitology Unit Research Guizetti Lab

Malaria research: Guizetti

the "Ganzetti" lab from left to right: Marie Jalovecka (guest scientist, alumna), Richard Thomson Luque (Postdoc), Vanessa Stürmer (HiWi, alumna), Marta Machado (PhD student), Wendy Straßburger (Msc student), Caroline Simon (PhD student), Severina Klaus (PhD student), Katharina Quadt (Postdoc, alumna), Yannik Voß (PhD student), Nicolas Lichti (intern, alumnus), Markus Ganter (group leader), Alexander Penning (MSc student, alumnus), Julien Guizetti (group leader).

Dr. Julien Guizetti (Group leader)

Anja Klemmer (PhD student)

Caroline Simon (PhD student)

Yannik Voß (PhD student)

We are always looking for motivated people to join our team!

We study the peculiar cell division mechanisms in Plasmodium blood stage parasites using advanced microscopy.

*** Further info under guizettilab.com ***

Research Interest

The Guizetti lab studies the unusual cell division mechanisms of the malaria parasite Plasmodium falciparum. Rapid mitotic divisions enable proliferation of the parasite in the human blood cells and contribute to disease severity. Even though mitosis in this parasite shows significant differences to what has been described in classical model organisms, it is poorly studied so far. We use super-resolution, electron, and live cell microscopy technologies combined with CRISPR/Cas9 genome editing to describe the dynamics and regulation of chromosomes, centromeres, and the nuclear envelope during division. Thereby we hope to uncover new targets within this essential pathway and contribute to the fight against malaria.

Guizetti J, Frischknecht F. (2021) Apicomplexans: A conoid ring unites them all. PLoS Biol. Mar 11;19(3):e3001105.

Barcons-Simon A, Cordon-Obras C, Guizetti J, Bryant JM, Scherf A. (2020) CRISPR Interference of a Clonally Variant GC-Rich Noncoding RNA Family Leads to General Repression of var Genes in Plasmodium falciparum. mBio. Jan 21;11(1)

Mehnert, A.K., Simon C.S., Guizetti, J. (2019). Immunofluorescence staining protocol for STED nanoscopy of Plasmodium-infected red blood cells. Mol Biochem Parasitol. 229, 47–52.

Müller, L. S. M., Cosentino, R. O., Förstner, K. U., Guizetti, J., Wedel, C., Kaplan, N., … Siegel, T. N. (2018). Genome organization and DNA accessibility control antigenic variation in trypanosomes. Nature 563(7729), 121–125

Zanghì, G., Vembar, S. S., Baumgarten, S., Ding, S., Guizetti, J., Bryant, J. M., … Scherf, A. (2018). A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection. Cell Reports 22(11), 2809–2817.

Bryant J.M., Regnault C., Scheidig-Benatar C., Baumgarten S., Guizetti J.*, Scherf A. (2017). CRISPR/Cas9 Genome Editing Reveals That the Intron Is Not Essential for var2csa Gene Activation or Silencing in Plasmodium falciparum. MBio 8(4). pii: e00729-17.

Guizetti, J.*, Barcons-Simon, A., Scherf, A. (2016). Trans-acting GC-rich non-coding RNA at var expression site modulates gene counting in malaria parasite. Nucleic Acids Res 44, 9710–9718.

Zhang, Q., Siegel, T. N., Martins, R. M., Wang, F., Cao, J., Gao, Q., Cheng, X., Jiang, L., Hon, C. C., Scheidig-Benatar, C., Sakamoto, H., Turner, L., Jensen, A. T., Claes, A., Guizetti, J., Malmquist, N. A., and Scherf, A. (2014). Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria. Nature 513, 431–435.

Guizetti, J., Martins, R. M., Guadagnini, S., Claes, A., and Scherf, A. (2013). Nuclear Pores and Perinuclear Expression Sites of var and Ribosomal DNA Genes Correspond to Physically Distinct Regions in Plasmodium falciparum. Eukaryot Cell 12, 697–702.

Guizetti, J., Schermelleh, L., Mantler, J., Maar, S., Poser, I., Leonhardt, H., Muller-Reichert, T., and Gerlich, D. W. (2011). Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments. Science 331, 1616–1620.

Guizetti, J., Mantler, J., Muller-Reichert, T., and Gerlich, D. W. (2010). Correlative time-lapse imaging and electron microscopy to study abscission in HeLa cells. Methods Cell Biol 96, 591–601.

Lacroix, B., van Dijk, J., Gold, N. D., Guizetti, J., Aldrian-Herrada, G., Rogowski, K., Gerlich, D. W., and Janke, C. (2010). Tubulin polyglutamylation stimulates spastin-mediated microtubule severing. J Cell Biol 189, 945–954.

Lekomtsev, S., Guizetti, J., Pozniakovsky, A., Gerlich, D. W., and Petronczki, M. (2010). Evidence that the tumor-suppressor protein BRCA2 does not regulate cytokinesis in human cells. J Cell Sci 123, 1395–1400.

Steigemann, P., Wurzenberger, C., Schmitz, M. H., Held, M., Guizetti, J., Maar, S., and Gerlich, D. W. (2009). Aurora B‑mediated abscission checkpoint protects against tetraploidization. Cell 136, 473–484.

 

Reviews

Guizetti, J.*, and Scherf, A. (2013). Silence, activate, poise and switch! Mechanisms of antigenic variation in Plasmodium falciparum. Cell Microbiol 15, 718–726.

Guizetti, J., and Gerlich, D. W. (2012). ESCRT-III polymers in membrane neck constriction. Trends Cell Biol 22, 133–140.

Guizetti, J., and Gerlich, D. W. (2010). Cytokinetic abscission in animal cells. Semin Cell Dev Biol 21, 909–916.

News - Guizetti group

Career Development Award

Julien Guizetti among the 11 awardees 2018

Our group leader Julien Guizetti is one of the winners of the "Career Development Award" 2018 of the "International Human Frontier Science Program Organisation" (HFSPO). The award ($300,000, spread over 3 years) is reserved for the most creative and accomplished among current HFSP fellowship holders and allows the award winners to return home or move to another member country to continue the frontier research they have begun in the life sciences.

How to get in touch:

Dr. Julien Guizetti

Centre for Infectious Diseases, Parasitology
Heidelberg University Hospital 
Im Neuenheimer Feld 324 (office & labs in INF 344, 2nd floor)
69120 Heidelberg, Germany

Tel. +49 6221 56-7877 (office) / -7882 (lab)

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