Biobank at the European Pancreas Centre (EPZ/Surgery Heidelberg)
Established by Prof. M. W. Büchler in 2002, the Biobank of European Pancreas Centre currently contains over 10.000 biospecimens and is a member of BMBH-Consortium.
The STARLims-managed collection of tissues and biofluids serves a broad spectrum of intra- and extramural research activities, particularly genome-wide screening programs. To accelerate clinical translation of our findings, we developed PancoBank-infrastructure which integrates two units.
The first unit is an Amazon-like platform enabling standardized web-based registration of the suitable samples, their management, quality assessment, and distribution. It allows to perform remote searches of the database by cooperating partners and provides them with HE-stained images and information about cellular composition of collected tissue samples—one of the most crucial aspects in the interpretation of the molecular profiles. This unit was developed in the framework of German-wide translational genomic network PaCaNet (NGFNPlus program funded by BMBF, www.ngfn-pacanet.de).
The second unit of the PancoBank utilizes developed by DKFZ iCHIP-technology (www.services.ichip.de), to store and to link PID-protected clinico-pathological records to the data delivered via own research activities or by various cooperating partners. The information includes qualitative (images) and quantitative data sets such as whole genome sequencing or measurements of mRNA, miRNA, SNPs, proteins and antibodies. These sets are used to elucidate molecular signatures associated with pancreatic pathogenesis and enabling stratification of the patients for novel treatments.
Heidelberg Cooperation partners
- EPZ-Outpatient clinic (Prof. Thilo Hackert, Dr. Christine Tjaden, Ulf Hinz)
- Institute of Pathology, University Hospital Heidelberg, Prof. Peter Schirmacher
- BMBH-team (Biomaterial Bank Heidelberg; Prof. Dr. Esther Herpel)
- DKFZ (German Cancer Research Center)
- Bioinformatics: Dr. M. Schlesner, Prof. B. Brors
- Genomics, proteomics, mRNA and miRNA profiling: Dr. Andrea Bauer and Dr. Jörg Hoheisel; Division of Functional Genome Analysis
- GWAS studies: Dr. Federico Canzian, Genomic Epidemiology Group
- Whole genome profiling: HIPO (Heidelberger Institute of Personalized Oncology; Peter Lichter) and NCT
- Noll EM, Eisen C, Stenzinger A, Espinet E, Muckenhuber A, Klein C, Vogel V,Klaus B, Nadler W, Rösli C, Lutz C, Kulke M, Engelhardt J, Zickgraf FM, Espinosa O, Schlesner M, Jiang X, Kopp-Schneider A, Neuhaus P, Bahra M, Sinn BV, Eils R, Giese NA, Hackert T, Strobel O, Werner J, Büchler MW, Weichert W, Trumpp A, Sprick MR. CYP3A5 mediates basal and acquired therapy resistance in different subtypes of pancreatic ductal adenocarcinoma. Nat Med. 2016 Mar;22(3):278-87.
- Wolpin, BM et al. Genome-wide association study identifies multiple susceptibility loci for pancreatic cancer. Nature Genetics 46, 994-1000 (2014)
- Schultz, NA et al. Diagnostic and Prognostic Impact of Circulating YKL-40, IL-6, and CA 19.9 in Patients with Pancreatic Cancer. PLoS One 8, e67059 (2013)
- Gress, TM et al. Differentiation of multiple types of pancreatico-biliary tumors by molecular analysis of clinical specimens. J Mol Med 90, 457-464 (2012)
- Bauer, AS et al. Diagnosis of pancreatic ductal adenocarcinoma and chronic pancreatitis by measurement of microRNA abundance in blood and tissue. PLoS One 7, e34151 (2012)
- Rizzato, C et al. Pancreatic cancer susceptibility Loci and their role in survival. PLoS One 6, e27921 (2011)
- Keller, A et al. Toward the blood-borne miRNome of human diseases. Nat Methods 8, 841-843 (2011)
- Alhamdani, MS et al. Single-step procedure for the isolation of proteins at near-native conditions from mammalian tissue for proteomic analysis on antibody microarrays. J Proteome Res 9, 963-971 (2010)
- Ceyhan, GO et al. Pancreatic Neuropathy and Neuropathic Pain - A comprehensive pathomorphological study of 546 Cases. Gastroenterology 136, 177-186 (2009)