Kliniken & Institute … Institute Zentrum für… Abteilung Virologie … Forschung Chlanda Lab

Research Group Chlanda

Research projects in our group focus on "Membrane Biology of Viral Infection".

Chlanda Lab – Group Photo

Projects

We are interested in studying how viruses interact with cellular membranes and lipids during infection. Many enveloped viruses such as influenza or Ebola must cross the cell membrane during entry and exit. To do so, viruses developed or hijacked fascinating protein machinery, which are able to remodel, fuse or cut membranes in processes dependent on specialized lipids. We use and further develop cryo-electron microscopy (cryo-EM) techniques in conjunction with other imaging methods as fluorescence microscopy and imaging mass spectrometry to solve the puzzle of viral-membrane interactions.

Influenza A Virus Entry and Membrane Fusion

Figure 1: Membrane fusion of influenza virus-like particles (VLPs) with liposomes (left). Influenza virus assembles and the plasma membrane of the host cell (right).

Influenza A virus (IAV) is a pleiomorphic, enveloped virus that enters the host cell either by endocytosis or macropinocytosis and fuses with the endosomal membrane in a Hemagglutinin (HA)-mediated process that occurs at low pH. Neither the structure of the HA fusion intermediates nor the spatial orientation of the post-fusion HA with respect to the fusion pore are known. We use cryo-ET and subtomogram averaging to further characterize the structure of HA fusion intermediates. In addition, we study viral fusion and disassembly in the cells close to their native state using cryo-CLEM, cryo-FIB/SEM and cryo-ET. This will allow to further investigate the role of host factors such as aggresome machinery, cytoskeleton, IFITMs proteins and lipids such as cholesterol on the membrane fusion and virion disassembly.

Structual Analysis of Ebola Virus Entry Intermediates

In Vitro and In Situ

Figure 2: Ebola virus-like particles are predominantly filamentous.

Ebola virus (EBOV) assembles into long filamentous virions (1–15 μm) at the plasma membrane, which upon release, enter epithelial cells by macropinocytosis. The negative single stranded RNA genome is coiled across a length of about 0.9 μm and protected by the nucleocapsid composed of the nucleoprotein (NP), VP35, VP40, and VP24 proteins. The Ebola fusion glycoprotein (GP) is proteolytically processed in the late endosome by low-pH sensitive cathepsin proteases to a 19 kDa fragment, which binds to Niemann-pick-C1 receptor (NPC1). The 19 kDa fragment bound to NPC1 together with yet unknown factor(s) is able to induce membrane fusion allowing the release of the genome. Both the disassembly of the filamentous virus and the mechanism underlying GP mediated membrane fusion in the endosomes are poorly understood processes and have not been structurally characterized. We use non-infectious EBOV VLPs, which are composed of five major structural proteins (GP, NP, VP40, VP35, and VP24) and are structurally similar to EBOV to study EBOV virus entry intermediates by cryo-CLEM, cryo-FIB/SEM and cryo-ET both in vitro and in living cells.

Spatial Lipidomics and Viral Infection

Figure 3: Correlative cryo-SIMS/ET.

Secondary ion mass spectrometry (SIMS) allows non-invasive imaging of chemically unmodified lipids with high chemical specificity. However, SIMS has so far been only performed on chemically fixed and dehydrated samples (sample preparation procedures known to severely alter membrane structure). In collaboration with Tom Wirtz (Luxembourg Institute of Science and Technology), we will apply SIMS to vitreous cryo-lamellas of the cells prepared by focused-ion beam milling to provide a spatial map of lipids in cellular organelles at native conditions. The lipid map will be subsequently correlated to the membrane structures observed by cryo-ET. We study lipids such as cholesterol which is crucial in host-pathogen interactions as well as in membrane trafficking.


Selected Publications

Complete Publication List (PubMed)

  • Chlanda, P., Mekhedov, E., Waters, H., Sodt, A., Schwartz, C., Nair, V., Blank, P.S., Zimmerberg, J. (2017) Palmitoylation contributes to membrane curvature in Influenza A virus assembly and hemagglutinin-mediated membrane fusion. J. Virol. doi: 10.1128/JVI.00947–17.
     
  • Chlanda, P., Krijnse Locker, J., (2017) The sleeping beauty kissed awake: new methods in electron microscopy to study cellular membranes. Biochemical Journal, DOI: 10.1042/BCJ20160990
     
  • Quemin, E.R.J.*, Chlanda, P.*, Sachse, M., Forterre, P., Prangishvili, D. and Krupovic, M., (2016) Eukaryotic-like virus budding in Archaea, MBio, doi: 10.1128/mBio.01439–16. (*Equal contribution)
     
  • Chlanda, P., Mekhedov, E., Waters, H., Schwartz, C.L., Fischer, E.R., Ryham, R.R., Cohen, F.S., Blank, P.S. and Zimmerberg, J. (2016), The hemifusion structure induced by Influenza virus hemagglutinin is determined by physical properties of the target membranes. Nature Microbiology, doi:10.1038/nmicrobiol.2016.50
     
  • Chlanda, P., Zimmerberg, J. (2016), Protein-lipid interactions critical to replication of the influenza A virus during infection. FEBS Lett., doi: 10.1002/1873–3468.12118
     
  • Chlanda, P., Schraidt, O., Kummer, S., Riches, J., Oberwinkler, H., Prinz, S., Kräusslich HG and Briggs, J.A.G. (2015), Structural analysis of the contributions of individual proteins to influenza assembly and morphology. J Virol, 89(17):8957–66.
     
  • Chlanda, P., Sachse, M. (2014), Cryo-Electron Microscopy of Vitreous Sections. Methods Mol Biol, 1117:193–214.
     
  • Krijnse Locker, J., Chlanda, P., Sachsenheimer, T., Brügger, B. (2013), Poxvirus membrane biogenesis: rupture not disruption. Cell Microbiol, 15(2):190–9.
     
  • Chlanda, P., Carbajal, M.A., Cyrklaff, M., Griffiths, G., Krijnse-Locker, J. Membrane rupture generates single open membrane sheets during vaccinia virus assembly. (2009), Cell Host Microbe, 6(1):81–90.
     
  • Cyrklaff, M., Linaroudis, A., Boicu, M., Chlanda, P., Baumeister, W., Griffiths, G., Krijnse-Locker, J. (2007), Whole cell cryo-electron tomography reveals distinct disassembly intermediates of vaccinia virus. PLoS ONE, 2(5):e420.

Group Members

Alumni

Former Lab Members

  • Benedikt Wimmer