In our Cardiac Sequencing Unit we use latest methods for short-read sequencing on a NovaSeq or MiniSeq (Illumina) and long-read sequencing on a GridIon or MinION (Oxford Nanopore). For automated library generation a MagnisDX (Agilent) is available. Methods to be applied include, but are not limited to e.g.:

• Whole genome sequencing
• Whole-exome sequencing
• Partial exome sequencing in diagnostic coverage (>2000x)
• miRNome sequencing
• Transcriptome sequencing
• Bisulfite sequencing
• Single-cell sequencing

Besides the wet-lab infrastructure, we also are well-equipped for downstream bioinformatics analyses. Most analyses run on a Unix cluster system with 768 CPUs and 3840 NVIDIA Tensor cores. All aspects of primary, secondary and tertiary evaluation can be processed using standard or individually tailored pipelines. Within the framework of the German Center for Cardiovascular Research (DZHK), this platform serves as “Shared Expertise”. If you have any questions, please feel free to contact us at any time.

Cardiac Sequencing Core Facility

•  NovaSeq 6000 (Illumina)

•  MiniSeq (Illumina)

•  GridIon (Oxford Nanopore)

•  MinIon (Oxford Nanopore)

 

 

 

•  Multi-node Compute-Cluster (CPU & GPU)

•  >2PB Mirrored Storage