Kliniken & Institute … Kliniken Neurologische Klinik … Neurologie und… Forschung Neurooncology Experimental…

EXPERIMENTAL NEUROONCOLOGY

OVERVIEW

The central interest of the research group is to understand how primary and metastatic tumors progress in the brain, and how this information can be used to improve therapies. The research focus lies on clinically relevant, but also basic questions in brain tumor research, but also other neurological diseases. To study the initiation, progression and therapeutic responses of diseases of the central nervous system, we have established refined animal models using in vivo two-photon microscopy in our DKFZ lab. This methodology allows studying brain cancer cell populations and their dynamic behavior over many months, including their cellular components, gene expression, blood vessels, glia cells, neurons, intercellular communications, and important physiological and therapeutical parameters like hypoxia, blood flow velocity, and vascular permeability. This unique approach makes it possible to investigate dynamic interactions of cells, and the key mechanisms in a live organism over long periods of time in high resolution. We currently pursue the following main research projects:

Prof. Dr. med. Frank Winkler

Arbeitsgruppenleiter (AG Experimental Neurooncology)


The role of tumor microtubes in brain tumor progression: We discovered that ultra-long and ultra-thin membrane extensions of astrocytoma (including glioblastoma) cells are highly relevant for tumor progression and resistance to therapies (Osswald Nature 2015). The resulting multicellular tumor network allows intensive intercellular communication, and better cellular homeostasis, which results in resistance to radiotherapy. In ongoing projects, we aim to better understand 1) whether tumor microtubes are also relevant for communication with cell types of the normal brain; 2) how tumor microtubes, and the functional network they form, can be optimally targeted by novel therapies – to break the notorious treatment resistance of many brain tumors; 3) whether and how epilepsy, a frequent problem for brain tumor patients, is related to these discoveries.

The role of cancer stem cells for brain tumor formation and growth.

Angiogenesis and invasion of gliomas: In cooperation projects with industry, we investigate how modern antiangiogenic therapies modulate growth and invasion of gliomas, and their response to chemo- and radiotherapy.

Brain metastases prevention: This novel approach, supported by results from own previous work (Kienast Nat Med 2010; Ilhan-Mutlu Mol Cancer Ther 2016), appears to be a clinically feasible concept for certain tumor entities. Prof. Winkler is Principal Investigator of the prevent_BM consortium, funded by the German Cancer Aid (Deutsche Krebshilfe), which consequently explores this exciting translational research concept as a novel strategy in Oncology.

Using in vivo two-photon microscopy, a laser beam was used to kill one glioblastoma cell (dashed circle) that is connected to other tumor cells with fine membrane tubes ("tumor microtubes"). The reaction of the tumor cell network to this loss is remarkable: a new tumor microtube is formed (arrowheads), a distant network cell divides, and the new nucleus (star) travels through the microtube to the very place where the dead network member was located. After 60 hours, the tumor cell network has repaired itself. Inhibition of this process is currently explored to decrease treatment resistance of brain tumors.

Team

Wiss. Mitarbeiter/-innen

SELECTED PUBLICATIONS

  • Winkler F, Wick W. Harmful networks in the brain and beyond. Science. 2018 Mar 9;359(6380):1100-1101
  • Osswald M, Jung E, Sahm F, Solecki G, Venkataramani V, Blaes J, Weil S, Horstmann H, Wiestler B, Syed M, Huang L, Ratliff M, Karimian Jazi K, Kurz FT, Schmenger T, Lemke D, Gommel M, Pauli M, Liao Y, Haring P, Pusch S, Herl V, Steinhauser C, Krunic D, Jarahian M, Miletic H, Berghoff AS, Griesbeck O, Kalamakis G, Garaschuk O, Preusser M, Weiss S, Liu H, Heiland S, Platten M, Huber PE, Kuner T, von Deimling A, Wick W, Winkler F.  Brain tumour cells interconnect to a functional and resistant network. Nature. 2015 Dec 3;528(7580):93-8
  • Weil S, Osswald M, Solecki G, Grosch J, Jung E, Lemke D, Ratliff M, Hänggi D, Wick W, Winkler F (2017). Tumor microtubes convey resistance to surgical lesions and chemotherapy in gliomas. Neuro Oncol. 2017 Oct 1;19(10):1316-1326
  • Jung E, Osswald M, Blaes J, Wiestler B, Sahm F, Schmenger T, Solecki G, Deumelandt K, Kurz FT, Xie R, Weil S, Heil O, Thomé C, Gömmel M, Syed M, Häring P, Huber PE, Heiland S, Platten M, von Deimling A, Wick W, Winkler F. Tweety-Homolog 1 Drives Brain Colonization of Gliomas. J Neurosci. 2017 Jul 19;37(29):6837-6850
  • Osswald M, Blaes J, Liao Y, Solecki G, Gömmel M, Berghoff AS, Salphati L, Wallin JJ, Phillips HS, Wick W, Winkler F. Impact of blood-brain barrier integrity on tumor growth and therapy response in brain metastases. Clin Cancer Res. 2016 Dec 15;22(24):6078-6087
  • von Baumgarten L, Brucker D, Tirniceru A, Kienast Y, Grau S, Burgold S, Herms J, Winkler F. Bevacizumab has differential and dose-dependent effects on glioma blood vessels and tumor cells. Clin Cancer Res. 2011 Oct 1;17(19):6192-205
  • Ilhan-Mutlu A, Osswald M, Liao Y, Goemmel M, Reck M, Miles D, Mariani P, Gianni L, Lutiger B, Nendel V, Strock S, Perez-Moreno PD, Thorsen F, von Baumgarten LD, Preusser M, Wick W, Winkler F. Bevacizumab prevents brain metastases formation in lung adenocarcinoma. Mol Cancer Ther. 2016 Apr;15(4):702-10
  • Kienast Y, von Baumgarten L, Fuhrmann M, Klinkert W, Goldbrunner R, Herms J, Winkler F. Real-time imaging reveals the single steps of brain metastasis formation. Nat Med. 2010 Jan;16(1):116-22
  • Winkler F, Kozin SV, Tong RT, Chae S, Booth MF, Garkavtsev I, Xu L, Hicklin DK, Fukumura D, di Tomaso E, Munn LL, RK Jain RK. Kinetics of vascular normalization by VEGFR2 blockade governs brain tumor response to radiation: Role of oxygenation, Angiopoietin-1, and matrix metalloproteinases. Cancer Cell. 2004 Dec;6(6):553-63